BioFire FilmArray Technology
Three Easy Steps to Faster Disease Diagnostics
- SETUP Inject hydration solution and an unprocessed sample.
- TEST Insert the pouch into the FilmArray unit and start the run.
- GET RESULT The software reports whether or not each pathogen is detected in the sample.
BioFire FilmArray Technology
The BioFire FilmArray pouch stores all the necessary reagents for sample preparation, reverse transcription-PCR, PCR, and detection in a freeze-dried format. Just inject hydration solution and an unprocessed sample, and the BioFire FilmArray System takes care of the rest.
During a run, the BioFire FilmArray System extracts and purifies all nucleic acids from the unprocessed sample. Next, the FilmArray performs a nested multiplex PCR. During the first stage, the system performs a single, large volume, multiplexed reaction. Finally, individual, single-plex second-stage PCR reactions detect the products from the first-stage PCR.
Using endpoint melting curve data, the BioFire FilmArray software automatically analyzes the result for each target. When the run is complete, the software reports whether or not each pathogen is detected in the sample. This information is printed in an automated response at the end of the test run.