BioSurveillance Systems
Food & Water Security Testing Systems
Hi-Res Melting for Mutation Discovery
	- LightScanner
	- LightScanner Master Mixes
	- LCGreen Plus Melting Dye
	- LightScanner Assays
Clinical Diagnostic System

Mutation Discovery Using High Resolution Melting

Mutations in PCR products are detected by the presence of heteroduplexes optimally detected by our LCGreen® Plus dye. Variations are identified by changes in the shape of the melting profile compared to a reference sample. Hi-Res Melting® (HRM) on the LightScanner® is also being used for mutation discovery in tumor samples, yielding information on mutations present in low allele fractions (as low as 5%) and allelic imbalances between tumor and matched normal samples.

Use the links below to learn how your research can get there faster with our complete line of products.

Instrument

LightScanner® System

Hi-Res Melting Instrument
96- or 384-well plate system
Results in less than 5 minutes
Software automatically analyzes each sample and defines the standards and variants

Chemistry

LightScanner Master Mix

A pre-optimized solution
specifically designed to deliver superior PCR performance for Hi-Res Melting applications on the LightScanner Instrument.

LCGreen® Plus Melting Dye

LCGreen dye is specifically designed for Hi-Res Melting curve analysis to detect DNA sequence variants (mutations, polymorphisms, etc).

Services

LightScanner Assays

Free assay design protocols that have been optimized for Hi-Res Melting.