Benefits from a Complete Product Solution
Instrument |
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Chemistry |
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Results in less than five minutes |
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Optimized Master Mix for superior PCR performance for Hi-Res Melting applications |
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Hi-Res Melting is non-destructive allowing samples to be recovered for further analysis |
Support |
Software |
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Obtain quick assistance through phone and online help desks |
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Software automatically analyses each sample and defines the standards and variants |
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Work directly with highly experienced scientists |
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Simple data management tools |
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Primer design software |
 LightScanner Software
Our comprehensive software package provides both assay design and data analysis modules. The primer design module streamlines assay design for Hi-Res Melting. The analysis software features Call-IT®, a patented scientific method where the software critically analyses each sample, defines and groups wild-types and variants. This software also provides users with simple data management tools.
High Resolution Melting References
The LightScanner Hi-Res Melting system has been used for over five years and has resulted in approximately 200 peer-reviewed publications, making it the most highly published Hi-Res Melting instrument. Here are a few select references for your review.
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De Koeyer D, Douglass K, Murphy A, Whitney S, Nolan L, Song Y, De Jong W (2010). Application of high-resolution DNA melting for genotyping and variant scanning of diploid and autotetraploid potato. Mol Breeding. 25, 67–90.
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Rolf H.A.M. Vossen, Emmelien Aten, Anja Roos, Johan T. den Dunnen. (2009). High-Resolution Melting Analysis (HRMA) - More than just sequence variant screening Human Mut. 30, 860-866.
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Parant JM, George SA, Pryor R, Wittwer CT, Yost HJ. (2009). A rapid and efficient method of genotyping zebrafish mutants. Dev Dyn. 238,3168-74.
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van der Stoep N, van Paridon CDM, Janssens T, Krenkova P, Stambergova A, Macek M, Matthijs G, Bakker E. (2009). Diagnostic guidelines for high-resolution melting curve (HRM) analysis: An interlaboratory validation of BRCA1 mutation scanning using the 96-well LightScanner. Human Mut. 30, 899-909.
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Aten E, White SJ, Kalf ME, Vossen RH, Thygesen HH, Ruivenkamp CA, Kriek M, Breuning MH, den Dunnen JT.(2008). Methods to detect CNVs in the human genome. Cytogenet Genome Res. 123, 313-21.
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Audrezet MP, Dabricot A, Le Marechal C, Ferec C. (2008). Validation of high-resolution DNA melting analysis for mutation scanning of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. J Mol Diagn. 10, 424-34.
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C. Voegele, S.V. Tavtigian, D. de Silva, Cuber Thomas, F. Le Calvez-Kelm. (2007). A Laboratory Information Management System (LIMS) for a high throughput genetic platform aimed at candidate gene mutation screening. Bioinformatics. 23, 2504-2506.
LightScanner and Computer Specifications
| Instrument Specifications |
| Size (w x d x h) |
7.8” (20 cm) x 16.5” (42 cm) x 31.8” (81 cm) |
| Weight |
25 kg / 55 lbs |
| Voltage Input |
100-240 VAC, +/-10%, 50-60 Hz with automatic detection and configuration, 2.5 A |
| Wattage |
800 W maximum including computer and monitor. |
| Cooling time |
95° to 60 °C (non-linear)
~ 180 seconds at ambient temperature 25 °C |
| Heating rate |
0.1 °C per second |
| Single run time |
15 minutes, including cool down |
| Certifications |
CE, UL (for Electrical Safety) |
| Sample Handling and Filter System |
| Sample format |
Standard 96- or 384-well trays |
| Reaction volume |
5-100 µl |
| Sample temperature range |
40° to 100 °C |
| Excitation filter |
470 +/- 20 nm |
| Emission filters |
510 and 600 nm |
| Computer and Software |
| LightScanner Software |
Accurately performs mutation scanning and genotyping by Hi-Res Melting curve analysis |
| Computer |
Pentium-based PC with Windows XP Professional, 17”- 1280 x 1024 graphics monitor and software. |
| Networking |
Ethernet connection allows export of data in standard spreadsheet format compatible with LIMS software.
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| Ordering Information |
LightScanner System
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96-Well Instrument |
384-Well Instrument |
LSCN-ASY-0011 |
LSCN-ASY-0001 |
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LightScanner® System
