The FilmArray reagent pouch stores all the necessary reagents for sample preparation, reverse transcription-PCR, PCR, and detection in a freeze-dried format. Prior to a run, the user injects hydration solution and unprocessed sample into the pouch. The FilmArray instrument does the rest.
The FilmArray Pouch
First, the FilmArray extracts and purifies all nucleic acids from the unprocessed sample. Next, the FilmArray performs a nested multiplex PCR. During the first-stage PCR, the FilmArray performs a single, large volume, massively multiplexed reaction. Last, individual singleplex second-stage PCR reactions detect the products from the first stage PCR.
Using endpoint melting curve data, the FilmArray software automatically generates a result for each target.